Translation factor with broad impact
Distinct XPPX sequence motifs induce ribosome stalling, which is rescued by the translation elongation factor EF-P
Peil, L., Starosta, A.L., Lassak, J., Atkinson, G., Virumäe, K., Spitzer, M., Tenson, T., Jung, K., Remme, J., Wilson, D.N. (2013)
During protein synthesis, ribosomes catalyze peptide-bond formation between amino acids with differing efficiency. We show that two or more consecutive prolines induce ribosome stalling, and that stalling strength is influenced by the amino acid preceding and following the prolines. In bacteria, the elongation factor EF-P efficiently rescues the ribosome stalling irrespective of the XPP or PPX motif.