The Service unit MSBioLMU subunit Metabolomics provides routinely GC-MS and LC-MS analyses of small molecules. To this end, the service unit employs several mass spectrometers and chromatographs; two GC-TOF-MS and one UPLC-ESI-QTOF combination. With this equipment pool a broad range of metabolites can be identified and quantified. E.g.: amino acids, organic acids, sugars, fatty acids, glucosinolates, indoles, phenylpropanoides, flavonoides, lipides and porphyrines.
The service includes sample measurements, advisory service in experimental design and data exploration. To analyse the data, several software tools and mass spectral libraries are used.
To ensure the degree of purity required for all consumables the sample preparation will be done exclusively in our laboratories. Therefore, we need, depending on the kind of analysis, 5 mg (dry weight) or 50 mg (fresh weight) sample material. Methods of extraction will be adapted to the customer needs. Samples must be sent on dry ice or lyophilised. Please check the Sample Preparation Advices and fill the Sample Submission Form.
Before sending any samples, establish contacts to the service unit to check the machine run time capacity and confirm the correct receipt of your samples.
GC-MS data sets are evaluated using device-specific programs, as well as, the TagFinder software tool. The TagFinder employs spectra and retention time indices (RI) of the Golm Metabolom Database (GMD) for metabolite identification. LC-ESI-QTOF-MS data sets are evaluated by MassLynx V4.1 and further freeware tools.
The service unit has two Pegasus HT GC-TOF-MS (Leco) equiped with an autosampler (automated derivatisation/injection) to perform gas chromatography. This ensures a high comparability of the derivatisation process combined with minimised time offset between sample preparation and measurement. Furthermore, both GC machines have two different injection ports to enable hot and cold injection. A 30 m VF-5 ms column + 10 m guard column (Agilent Technologies) is routinely used.
LC analyses are performend on a UPLC-QTOF combination (Premier, Waters). The QTOF has an ESI source and different types of columns are available.
Currently we charge € 20 per sample for a routine analysis.